Journal: Oncotarget

Article Title: Human monocyte-derived dendritic cells exposed to hyperthermia show a distinct gene expression profile and selective upregulation of IGFBP6

doi: 10.18632/oncotarget.18338

Figure Lengend Snippet: A ., B. The dot plots, obtained by plotting the area of the cells (x-axis) vs the aspect ratio ( i.e. the ratio between length and height) parameter (y-axis), displaying the typical distribution of DCs in presence (A) or in absence (B) of permeabilization, show that permeabilization did not alter cell viability. C. , D. , E. , F. The percentage of cells expressing IGFBP-6 after exposure at 39°C at different times and the relative percentage of mean fluorescence intensity (MFI) of cells exposed at 39°C towards cells exposed at 37°C (considered as 100%) were analyzed by flow cytometry in the presence (C and D, respectively) or absence of permeabilization (E and F, respectively). No staining was seen with controls using either secondary antibody alone or isotype control rabbit IgG polyclonal antiserum (not shown). Each bar represents mean ±SEM of four experiments. * p < 0.05; ** p < 0.001. Statistical comparisons were made using unpaired Student’s t-test.

Article Snippet: In order to assess for the specificity of the IGFBP-6 chemotactic effect, we pre-incubated IGFBP-6 at the highest dose with a rabbit IgG anti-human C-terminal IGFBP-6 antibody (Abcam, Cambridge, UK) or with an irrelevant rabbit IgG antibody (anti-human HVCN1, Abcam, Cambridge, UK) in a weight ratio (IGFBP-6/antibody) of 1:6 for 30 min at room temperature, before adding the mixture in the lower compartment.

Techniques: Expressing, Fluorescence, Flow Cytometry, Staining

Journal: PLoS ONE

Article Title: Unique transcriptomic landscapes identified in idiopathic spontaneous and infection related preterm births compared to normal term births

doi: 10.1371/journal.pone.0225062

Figure Lengend Snippet: A. DKK1 , IGFBP1 , IGFBP2 , and IGFBP6 localization the syncytiotrophoblast in the control term births with increased expression in isPTB samples. Images are taken at 40x magnification and scale bar = 50um. B. QPCR validation of the upregulation of DKK1 , IGFBP1 , IGFBP2 , and IGFBP6 in isPTB vs TB samples. C . QPCR validation of hypermaturity signature genes TNC and C3 in isPTB vs TB samples. Student’s two-tailed T-test was utilized for statistical analyses and error bars represent standard deviation.

Article Snippet: Primary antibodies generated in rabbit sera were diluted in phosphate buffered saline (PBS): DKK1 (1:20, GeneTex GTX40056), IGFBP1 (1:50, GeneTex GTX31149), IGFBP2 (1:100, abcam ab227881), and IGFBP6 (1:100, Sigma HPA008005).

Techniques: Expressing, Two Tailed Test, Standard Deviation